Proper refrigeration is critical for many probiotics, both before and after they are purchased. In fact, in 2009 the majority (85%) of probiotics selected for testing by ConsumerLab.com did not contain their listed amounts of organisms and, as ConsumerLab.com later learned, improper shipping and warehousing by distributors and retailers appears to have been at least partly to blame. Fortunately, ConsumerLab.com found better results in early 2012 (17% of products failed testing), probably due to improvements in refrigeration procedures by several companies.
Many probiotic bacteria are sensitive to heat and moisture. Heat can kill organisms and moisture can activate them within pills, only to die due to lack of nutrients and a proper environment. These products should ideally be refrigerated.
Probiotics with freeze-dried organisms in appropriate packaging to prevent moisture, such as blister packs, generally do not need refrigeration. They also have longer shelf-lives than products containing live organisms, such as liquid products (which must be refrigerated). Probiotic yeast and some of the spore-forming bacteria, such as Bacillus coagulans, generally do not require refrigeration.
If you are purchasing a probiotic with a label that suggests or requires it be refrigerated, be sure your retailer has kept it refrigerated. If you order the product by mail, such as from an online retailer, be sure it is shipped overnight or with refrigerated shipping to minimize exposure to extreme heat.
Once you get the product, be sure to follow the manufacturer's recommendations. If in doubt, refrigerate.
For more information about probiotics and ConsumerLab.com's latest tests and comparisons, see the Product Review of Probiotic Supplements.